GRAM-NEGATIVE BACTERIA
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Context
- Recently, a multi-centre survey carried out by the ICMR to spot the trend in antibiotic resistance across the country showed Acinetobacter baumannii, a gram-negative bacteria known to cause infection in the blood, urinary tract and lungs among others, was resistant to high-end antibiotics.
Gram Staining
- Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram-positive and Gram-negative groups by coloring these cells red or violet.
- Gram-positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with.
- Alternatively, Gram-negative bacteria stain red, which is attributed to a thinner peptidoglycan wall, which does not retain the crystal violet during the decoloring process.
GRAM NEGATIVE VS GRAM POSITIVE BACTERIA
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