Source: Hindu
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Focus Area |
Feature |
Conservation and Health Initiatives |
βPreserve indigenous cattle breeds using NGS. βNew-generation vaccines against livestock diseases (e.g., brucellosis). |
Industry Collaboration and Innovation |
βBoost livestock economy through food security, vaccines, and diagnostics. βDevelop bio-scaffolds and a 3D model for TB research. |
R&D |
βIdentify TB resistance in cattle; explore CRISPR for productivity. βPromote sustainable protein and bacteriophage alternatives. βNIAB’s R&D efforts align with the BioE3 policy to boost bio-manufacturing and position India as a global leader in biotechnology. |
Diagnostics and Sustainable Farming |
βDevelop kits for brucellosis, mastitis, and hormone detection. βMILAN (Meeting of Livestock Farmers) showcases sustainable farming. βUse aquatic weed and yeast protein to cut emissions. |
Alternative Nutrition and Feed |
βAquatic Weed: Being introduced as potential livestock feed. βYeast-Derived Protein: Substitution in regular feed formulations to boost productivity. |
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The DNA fragments in the library need to be multiplied to ensure a strong enough signal during sequencing.
All DNA fragments are sequenced at the same time using an NGS machine.
Read basics of Genome Sequencing: https://www.iasgyan.in/daily-current-affairs/10000-genome-project-completed#:~:text=Importance%3A%20Genome%20sequencing%20enables%20scientists,diseases%2C%20and%20develop%20personalized%20treatments.
Read about DNA: https://www.iasgyan.in/daily-current-affairs/human-genome-project
Category |
Whole Genome Sequencing (WGS) |
Targeted Sequencing: Exome Sequencing |
Targeted Sequencing: Gene or Region-Specific Panels |
|
Description |
Sequencing the entire genome |
Sequencing only exons (protein-coding regions) |
Sequencing regions of interest such as disease-associated genes or genomic hotspots |
|
Pros |
βMost comprehensive genome coverage βDetects widest range of features: indels, structural and copy number variants, regulatory elements βNo bias from PCR amplification or probe hybridization βBest for discovery research |
β1% of human genome, less data to analyze than WGS βFaster workflow than WGS βMultiplexing small number of samples βMedium sample input (50 ng–1 μg depending on library prep method) |
β Highly flexible, customizable designs βData is focused specifically on regions/genes of interest βLowest sample input (10 ng) βMultiplexing large numbers of samples βBetter for detecting rare alleles |
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Cons |
βGenerates a lot of potentially unnecessary data from non-coding/non-functional regions βData is very complicated βMultiplexing usually not possible |
βOnly provides data on exons (may miss functionally relevant variants) βMay include extra data not needed for small gene studies |
βOnly provides data on targeted regions (may miss relevant variants if not in design) |
|
Speed/Return of Results |
Slowest |
Medium |
Fastest |
|
Cost |
$$$ |
$$ |
$ |
|
Data Volume |
Largest |
Medium |
Smallest |
|
When to Use |
βComplete coverage of genome needed βDe novo assembly βDiscovery of unknown genomic variants causing a disease βAneuploidy detection (preimplantation genetic testing) |
βDisease-specific research projects βClinical sequencing
|
βClinical sequencing βDisease-specific research projects βInherited disease βOncology βImmune repertoire βLiquid biopsy |
RNA type |
Function |
Messenger RNA |
Codes for protein |
Ribosomal RNA |
Translation |
Transfer RNA |
Translation |
Small nuclear RNA |
Splicing and other functions |
Small nucleolar RNA |
Nucleotide modification of RNAs |
Small Cajal body-specific RNA |
Type of snoRNA; nucleotide modification of RNAs |
Long noncoding RNA |
Regulation of gene transcription; epigenetic regulation |
MicroRNA |
Gene regulation |
Piwi-interacting RNA |
Transposon defense |
Small interfering RNA |
Gene regulation |
RNA sequencing method |
Description and benefits |
Total RNA |
βWhole transcriptome analysis to examine coding and noncoding RNA simultaneously; suitable for novel discovery. βMore throughput intensive to achieve high enough coverage for discovery. βPotential inefficiencies and bias due to different sequencing lengths. |
mRNA sequencing |
βAble to identify novel and known content |
smRNA sequencing |
βIsolation of small RNA to focus study on noncoding RNA to identify novel and known content such as microRNA (miRNA) |
Targeted RNA sequencing |
βSequencing specific transcripts of interest to focus efforts and lower cost to analyze specific genes of interest. |
Sources:
PRACTICE QUESTION Q: Consider the following statements regarding types of RNA:
Which of the above statements is/are correct? a) 1 only
Answer: c) Explanation: 1st statement is correct: PIWI-interacting RNAs (piRNAs) of 21–35 nucleotides in length silence transposable elements, regulate gene expression and fight viral infection. 2nd statement is correct: microRNA is the name of a family of molecules that helps cells control the kinds and amounts of proteins they make. That is, cells use microRNA to help control gene expression. Molecules of microRNA are found in cells and in the bloodstream. |
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